RT Journal Article
SR Electronic
T1 Relationship Between N-linked Oligosaccharide Chains of Human Serum Immunoglobulin G and Serum Tumor Markers with Non-small Cell Lung Cancer Progression
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4293
OP 4297
VO 26
IS 6B
A1 KANOH, YUHSAKU
A1 OHARA, TADASHI
A1 MASHIKO, TAKAOMI
A1 ABE, TADASHI
A1 MASUDA, NORIYUKI
A1 AKAHOSHI, TOHRU
YR 2006
UL http://ar.iiarjournals.org/content/26/6B/4293.abstract
AB Background: It has been demonstrated that increased expression of the sialyl Lex sugar chains on cancer cells influences cellular adhesion to vascular-endothelial cells. Therefore, it was thought that alterations in the sugar chain structure of E-cadherin, a calcium dependent adhesion molecule, influence the metastasis of cancer cells. Materials and Methods: In this study, N-linked oligosaccharides of human serum immunoglobulin G (IgG) were analyzed in 12 patients with non-small cell lung cancer (NSCLC) (6 localized cancer: 3 adenocarcinomas and 3 squamous cell carcinomas; 6 metastatic cancer: 3 adenocarcinomas and 3 squamous cell carcinomas) and 10 healthy controls using fluorophore-associated carbohydrate electrophoresis (FACE). The relationship between changes in sugar chain structure and serum concentrations of carcinoembryonic antigen (CEA) and cytokeratin 19 fragment (CYFRA21-1) were evaluated. CEA levels in the sera were determined using an enzyme immunoassay, and CYFRA21-1 levels were determined using an enzyme chemiluminescent immunoassay. Results: Fr1 (monogalactosyl IgG oligosaccharides) and Fr2 (digalactosyl IgG oligosaccharides) decreased, while Fr4 (agalactosyl IgG oligosaccharides) significantly increased (p<0.01-0.05) with NSCLC progression. The Fr4/Fr1+Fr2 ratio increased with NSCLC progression, and the ratios in localized and metastatic NSCLC were significantly higher than in healthy controls (p<0.01 and p<0.01, respectively). There was a strong correlation between serum CEA levels and Fr4 (r=0.91) and a significant correlation between serum CEA levels and the Fr4/Fr1+Fr2 ratio (r=0.83, p<0.05) in patients with lung adenocarcinoma. There was a significant correlation between serum CYFRA21-1 levels and Fr4 (r=0.88, p<0.001) and a positive correlation between serum CYFRA21-1 levels and the Fr4/Fr1+Fr2 ratio (r=0.38) in patients with lung squamous cell carcinoma. Conclusion: The analysis of serum IgG N-linked oligosaccharide chain structures by FACE may be an auxiliary indicator of serum tumor markers for monitoring NSCLC progression. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved