RT Journal Article
SR Electronic
T1 Circulating Tumor Cells With Epithelial–to–mesenchymal Transition Phenotypes Associated With Inferior Outcomes in Primary Breast Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1829
OP 1837
DO 10.21873/anticanres.13290
VO 39
IS 4
A1 MICHAL MEGO
A1 MARIAN KARABA
A1 GABRIEL MINARIK
A1 JURAJ BENCA
A1 JURISOVA SILVIA
A1 TATIANA SEDLACKOVA
A1 DENISA MANASOVA
A1 KATARINA KALAVSKA
A1 DANIEL PINDAK
A1 MASSIMO CRISTOFANILLI
A1 JAMES M. REUBEN
A1 JOZEF MARDIAK
YR 2019
UL http://ar.iiarjournals.org/content/39/4/1829.abstract
AB Background/Aim: Circulating tumor cells (CTCs) comprise a heterogeneous population of cancer cells with different clinical and biological value. The aim of this study was to evaluate the prognostic value of CTCs with an epithelial-mesenchymal transition (EMT) phenotype in primary breast cancer (PBC) patients. Patients and Methods: This study included 427 primary breast cancer patients. RNA extracted from CD45-depleted peripheral blood mononuclear cell (PBMCs) was evaluated for the expression of EMT transcription factors (TWIST1, SNAIL1, SLUG, ZEB1) by quantitative real time polymerase chain reaction (qRT-PCR). Results: In total, CTC EMT was detected in 77 (18.0%) patients. Patients without detectable CTC EMT in peripheral blood had significantly longer disease-free survival than patients with detectable CTC EMT. The prognostic value of CTC EMT was demonstrated in all subgroups of patients. Conclusion: CTCs with an EMT phenotype have a prognostic value in primary breast cancer.