PT  - JOURNAL ARTICLE
AU  - MYUNG, DAE-SEONG
AU  - OH, HYUNG-HOON
AU  - KIM, JE-SEONG
AU  - LIM, JAE-WOONG
AU  - LIM, CHAE-JUNE
AU  - GIM, SEUNG-EON
AU  - SEO, YOUNG-EUN
AU  - MA, KEON-YOUNG
AU  - YOU, GA-RAM
AU  - IM, CHAN MUK
AU  - HAN, BORA
AU  - SEO, YOON-JIN
AU  - PARK, SUN-YOUNG
AU  - PARK, YOUNG-LAN
AU  - JOO, YOUNG-EUN
TI  - Cytochrome P450 Family 46 Subfamily A Member 1 Promotes the Progression of Colorectal Cancer by Inducing Tumor Cell Proliferation and Angiogenesis
AID  - 10.21873/anticanres.16689
DP  - 2023 Nov 01
TA  - Anticancer Research
PG  - 4915--4922
VI  - 43
IP  - 11
4099  - http://ar.iiarjournals.org/content/43/11/4915.short
4100  - http://ar.iiarjournals.org/content/43/11/4915.full
SO  - Anticancer Res2023 Nov 01; 43
AB  - Background/Aim: Cytochrome P450 family 46 subfamily A member 1 (CYP46A1) has been implicated in the development and progression of various cancers. This study aimed to analyze the expression of CYP46A1, examining its relationship with oncogenic behaviors, and determining its prognostic implications in colorectal cancer (CRC). Materials and Methods: A total of 225 patients with CRC who underwent curative surgical resection were examined using paraffin-embedded tissue blocks and subjected to tumor-specific survival analysis. The expression of CYP46A1 was assessed in CRC tissues through reverse transcription-polymerase chain reaction, western blotting, and immunohistochemistry. The CRC cells’ apoptosis, proliferation, angiogenesis, and lymphangiogenesis were analyzed using terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays, alongside immunohistochemical staining for Ki-67, CD34, and D2-40 antibodies. Results: CYP46A1 expression was found to be up-regulated in CRC tissues compared to normal colorectal mucosa. Such expression was significantly associated with advanced stage, deeper tumor invasion, lymph node metastasis, distant metastasis, and decreased survival. Furthermore, the mean Ki-67 labeling index and microvessel density values in CYP46A1-positive tumors were significantly elevated compared to CYP46A1-negative tumors. However, there was no discernible correlation between CYP46A1 expression and either the apoptotic index or lymphatic vessel density value. Conclusion: CYP46A1 promotes CRC progression, specifically through the induction of tumor cell proliferation and angiogenesis. The insights provided may hold potential implications for future therapeutic interventions targeting CYP46A1.