TY - JOUR T1 - Antigens Recognized by IgG Derived from Tumor-infiltrating B Lymphocytes in Human Lung Cancer JF - Anticancer Research JO - Anticancer Res SP - 3607 LP - 3611 VL - 26 IS - 5A AU - MANABU YASUDA AU - MAKIKO MIZUKAMI AU - TAKESHI HANAGIRI AU - YOSHIKI SHIGEMATSU AU - TAKASHI FUKUYAMA AU - YOSHIKA NAGATA AU - TETSUYA SO AU - YOSHINOBU ICHIKI AU - MASAKAZU SUGAYA AU - MITSUHIRO TAKENOYAMA AU - KENJI SUGIO AU - KOSEI YASUMOTO Y1 - 2006/09/01 UR - http://ar.iiarjournals.org/content/26/5A/3607.abstract N2 - Background: Lung cancer tissues are often infiltrated by B lymphocytes, but it is not clear whether these infiltrations represent tumor-specific immune response or a nonspecific reaction. Materials and Methods: The serological analysis of recombinant cDNA expression libraries (SEREX) were previously modified using a severe combined immuno-deficient (SCID) mice model engrafted with fresh human lung cancer. Here, a panel of antigens recognized by tumor-infiltrating B lymphocytes (TIB) in human lung cancer were characterized. Results: The modified SEREX analysis identified 22 distinct antigens in a large cell carcinoma of the lung. Sequence analysis and real time-PCR analysis showed that 55% of isolated antigens were overexpressed in tumor tissues and 9% had mutation. Conclusion: The results of this study indicate that the humoral immune response of TIB in lung cancer patients can be detected in the xenotransplanted SCID mouse model and our modification shows high sensitivity and specificity for identification of tumor antigens. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -