%0 Journal Article %A XIANJUN QU %A YUNXIA YUAN %A WENFANG XU %A MINGHUI CHEN %A SHUXIANG CUI %A HONG MENG %A YAN LI %A MASATOSHI MAKUUCHI %A MUNEHIRO NAKATA %A WEI TANG %T Caffeoyl Pyrrolidine Derivative LY52 Inhibits Tumor Invasion and Metastasis via Suppression of Matrix Metalloproteinase Activity %D 2006 %J Anticancer Research %P 3573-3578 %V 26 %N 5A %X Background: LY52 is a caffeoyl pyrrolidine derivative designed to fit and extend into the active pocket of matrix metalloproteinase (MMP). In this study, the effects of LY52 on MMP-2 and MMP-9 activities and tumor invasion and metastasis were examined. Materials and Methods: MMP expression in SKOV3 cells was analyzed by gelatin zymography. The anti-invasion and anti-metastasis abilities of LY52 were evaluated with penetration of SKOV3 cells through Matrigel-coated membrane in vitro and pulmonary metastasis of Lewis lung carcinoma in mice, respectively. Results: LY52 significantly blocked the proteolytic activity of gelatinase. Gelatin zymography revealed that MMP-2 and MMP-9 expressions in SKOV3 cells were reduced in the presence of LY52. LY52 also suppressed SKOV3 cell invasion in vitro. Furthermore, a significant inhibition of pulmonary metastasis of Lewis lung carcinoma cells was observed in LY52-administrated mice. Conclusion: LY52 might suppress invasion and metastasis of carcinoma cells via inhibition of MMP-2 and MMP-9 proteolytic activities. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved %U https://ar.iiarjournals.org/content/anticanres/26/5A/3573.full.pdf