RT Journal Article
SR Electronic
T1 Effect of 2-Deoxy-D-glucose on Various Malignant Cell Lines <em>In Vitro</em>
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3561
OP 3566
VO 26
IS 5A
A1 ZHANG, XIAO DONG
A1 DESLANDES, EDWIGE
A1 VILLEDIEU, MARIE
A1 POULAIN, LAURENT
A1 DUVAL, MARILYNE
A1 GAUDUCHON, PASCAL
A1 SCHWARTZ, LAURENT
A1 ICARD, PHILIPPE
YR 2006
UL http://ar.iiarjournals.org/content/26/5A/3561.abstract
AB Background: 2-Deoxy-D-glucose (2-DG) is an analog of glucose that is preferentially captured by tumors and is accumulated in transformed cells, because the phosphorylated molecule (2-DG-6P) cannot be metabolized or diffused outside the cells. Targeted with a fluorine atom, 18F-DG is currently used to visualize malignant tumors (PET scan). Although cancer cells have been reported to be strongly dependent on glycolysis (Warburg effect), very few reports have studied the inhibitory effects of 2-DG on cancer. Materials and Methods: Our objective was to study, in a large panel of human malignant cells of various origins (ovarian, squamous, cerebral, hepatic, colonic and mesothelial), if the inhibitory activity of 2DG against tumor growth could be considered a general phenomenon and to determine its effect on the cell cycle. Results: Four types of response in the different cell lines were observed when cells were cultured in the presence of 2-DG (5 mM) continuous exposure: proliferation slow down; proliferation arrest without signs of apoptosis; strong cell cycle arrest accompanied by moderate apoptosis induction; massive apoptosis. Conclusion: 2-DG appears as an interesting new therapeutic agent against cancer in vitro, and should be tested in in vivo studies. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved