PT  - JOURNAL ARTICLE
AU  - XIAO DONG ZHANG
AU  - EDWIGE DESLANDES
AU  - MARIE VILLEDIEU
AU  - LAURENT POULAIN
AU  - MARILYNE DUVAL
AU  - PASCAL GAUDUCHON
AU  - LAURENT SCHWARTZ
AU  - PHILIPPE ICARD
TI  - Effect of 2-Deoxy-D-glucose on Various Malignant Cell Lines <em>In Vitro</em>
DP  - 2006 Sep 01
TA  - Anticancer Research
PG  - 3561--3566
VI  - 26
IP  - 5A
4099  - http://ar.iiarjournals.org/content/26/5A/3561.short
4100  - http://ar.iiarjournals.org/content/26/5A/3561.full
SO  - Anticancer Res2006 Sep 01; 26
AB  - Background: 2-Deoxy-D-glucose (2-DG) is an analog of glucose that is preferentially captured by tumors and is accumulated in transformed cells, because the phosphorylated molecule (2-DG-6P) cannot be metabolized or diffused outside the cells. Targeted with a fluorine atom, 18F-DG is currently used to visualize malignant tumors (PET scan). Although cancer cells have been reported to be strongly dependent on glycolysis (Warburg effect), very few reports have studied the inhibitory effects of 2-DG on cancer. Materials and Methods: Our objective was to study, in a large panel of human malignant cells of various origins (ovarian, squamous, cerebral, hepatic, colonic and mesothelial), if the inhibitory activity of 2DG against tumor growth could be considered a general phenomenon and to determine its effect on the cell cycle. Results: Four types of response in the different cell lines were observed when cells were cultured in the presence of 2-DG (5 mM) continuous exposure: proliferation slow down; proliferation arrest without signs of apoptosis; strong cell cycle arrest accompanied by moderate apoptosis induction; massive apoptosis. Conclusion: 2-DG appears as an interesting new therapeutic agent against cancer in vitro, and should be tested in in vivo studies. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved