PT  - JOURNAL ARTICLE
AU  - ENDO, IHIRO
AU  - AMATYA, VISHWA JEET
AU  - KUSHITANI, KEI
AU  - KAMBARA, TAKAHIRO
AU  - NAKAGIRI, TETSUYA
AU  - AOE, KOHEI
AU  - TAKESHIMA, YUKIO
TI  - FOXM1 Promotes Mesothelioma Cell Migration and Invasion <em>via</em> Activation of SMAD Signaling
AID  - 10.21873/anticanres.16583
DP  - 2023 Sep 01
TA  - Anticancer Research
PG  - 3961--3968
VI  - 43
IP  - 9
4099  - http://ar.iiarjournals.org/content/43/9/3961.short
4100  - http://ar.iiarjournals.org/content/43/9/3961.full
SO  - Anticancer Res2023 Sep 01; 43
AB  - Background/Aim: Forkhead box M1 (FOXM1) is a transcription factor closely associated with various human malignancies and is considered an attractive target for cancer therapy. Mesothelioma is a malignancy primarily due to asbestos exposure and certain genetic factors, requiring a better understanding of tumorigenesis for improved treatment. Asbestos-exposed human mesothelial cells have been reported to up-regulate FOXM1 expression in a dose-dependent manner. Materials and Methods: FOXM1 expression was evaluated in mesothelioma tissues and cell lines. FOXM1 small interfering RNA was transfected into mesothelioma cell lines to analyze its biological functions and regulatory mechanisms. Results: FOXM1 was over-expressed in mesothelioma tissues and cell lines. Knock-down of FOXM1 in mesothelioma cell lines inhibited cell proliferation, migration, and invasion. These results suggest that up-regulation of FOXM1 expression promotes mesothelioma tumorigenesis and progression. We previously reported that insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) promotes the proliferation, migration, and invasion of mesothelioma cell lines. In this study, IGF2BP3 knock-down suppressed FOXM1 expression in mesothelioma cell lines. Our results suggest that IGF2BP3, an upstream regulator, contributes to increased FOXM1 expression. Furthermore, IGF2BP3 and FOXM1 knock-down suppressed SMAD signaling by inhibiting SMAD2/3 phosphorylation in mesothelioma cell lines. Conclusion: IGF2BP3/FOXM1 promotes mesothelioma cell migration and invasion via SMAD signaling, highlighting IGF2BP3/FOXM1 as a potential target for mesothelioma treatment.