RT Journal Article SR Electronic T1 Apoptosis-related Prognostic Factors in Advanced Colorectal Cancer Determined Using Tissue Microarrays JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 3935 OP 3942 DO 10.21873/anticanres.16581 VO 43 IS 9 A1 OZAKI, KOSUKE A1 SASAKI, KAZUHITO A1 ABE, HIROYUKI A1 NOZAWA, HIROAKI A1 MURONO, KOJI A1 EMOTO, SHIGENOBU A1 USHIKU, TETSUO A1 ISHIHARA, SOICHIRO YR 2023 UL http://ar.iiarjournals.org/content/43/9/3935.abstract AB Background/Aim: Cancer cells evade apoptosis in colorectal cancer (CRC); however, overlap between apoptosis and poor prognosis marker proteins in the invasive front of tumors has not been reported. Here, we aimed to clarify the relationship between apoptosis, apoptosis-related protein expression, and prognosis in the central and invasive front regions of CRC using tissue microarrays. Patients and Methods: Data of 207 patients with pathological stage 3 CRC, who underwent radical surgery between October 2010 and November 2014, were retrospectively reviewed. We assessed apoptosis using M30 CytoDEATH, CD163, and p53 immunostaining in tumor sections in the center and invasive front using tissue microarrays and correlated the results with the survival outcomes. Results: M30 CytoDEATH staining was negative; 134 cases (64.7%) were apoptosis-negative in the center and 103 (49.8%) were apoptosis-negative at the invasive front. CD163 positivity was observed in 16 cases (7.8%) in the center and in 36 cases (17.6%) at the invasive front; p53 positivity was observed in 33 (15.9%) and 64 (30.9%) cases in the center and invasive front, respectively. CD163 and p53 expression was not associated with survival outcomes; however, the apoptosis-negative group at the invasive front had significantly poorer survival outcomes (overall survival: p=0.044, relapse-free survival: p=0.001). We identified cases with a poor prognosis by combining apoptosis and CD163 expression. Conclusion: A lower apoptosis percentage at the invasive front is associated with a poorer prognosis. CRC cases with a poor prognosis can be identified by evaluating apoptosis and CD163 expression in the invasive front.