TY - JOUR T1 - Kinetics of MMP-1 and MMP-3 Produced by Mast Cells and Macrophages in Liver Fibrogenesis of Rat JF - Anticancer Research JO - Anticancer Res SP - 3517 LP - 3526 VL - 26 IS - 5A AU - WON-IL JEONG AU - SUN-HEE DO AU - DA-HEE JEONG AU - IL-HWA HONG AU - JIN-KYU PARK AU - KI-MI RAN AU - HAI-JIE YANG AU - DONG-WEI YUAN AU - SOON-BOK KIM AU - MYUNG-SOOK CHA AU - KYU-SHIK JEONG Y1 - 2006/09/01 UR - http://ar.iiarjournals.org/content/26/5A/3517.abstract N2 - Background: The involvement of whether macrophages and mast cells in the kinetics of matrix metalloproteinases-1 (MMP-1), MMP-3 and MMP-9, tissue inhibitors of metalloproteinases-1 (TIMP-1) and TIMP-2 in hepatic fibrosis/cirrhosis was examined. Materials and Methods: The MMPs and TIMPs were examined by histopathology, immunohistochemistry and immunoblotting using carbon tetrachloride-induced fibrotic rat liver. Results: MMP-1 increased in proportion to the development of fibrosis and reached maximum at week 14. In the first four weeks, MMP-3 expression was mainly observed in many hepatocytes. At week 8, the macrophages in the fibrous septa, as well as hepatocytes, expressed MMP-3. TIMP-1 and -2 progressively increased throughout the experimental periods. The MMP-1 expression in the mast cells, however, did not decrease the degree of liver cirrhosis. Conclusion: MMP-1, TIMP-1 and -2 expressions increased in the late stages of fibrosis and cirrhosis. During recovery, the MMP-3 expression of macrophages greatly increased in the unresolved fibrous septa. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -