RT Journal Article
SR Electronic
T1 Cyclin-dependent Kinase (CDK) Inhibitor Olomoucine Enhances γ-Irradiation-induced Apoptosis and Cell Cycle Arrest in Raji Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3493
OP 3498
VO 26
IS 5A
A1 BOUTIS, ANASTASIOS
A1 PAPAZISIS, KONSTANTINOS
A1 PISTEVOU-GOMPAKI, KYRIAKI
A1 LAMBROPOULOS, ALEXANDROS
A1 SOFRONIADIS, IOANNIS
A1 PAPAGEORGIOU, ATHANASIOS
A1 DESTOUNI, EVANGELIA
A1 KORTSARIS, ALEXANDROS
YR 2006
UL http://ar.iiarjournals.org/content/26/5A/3493.abstract
AB Gamma-irradiation leads to apoptosis and cell cycle arrest in eukaryotic cells. Olomoucine is a novel purine analog acting as a cyclin-dependent kinase inhibitor. The effects of olomoucine in gamma-irradiation mediated cell growth inhibition and apoptosis were studied in the Raji cell line (Burkitt's lymphoma). Gamma-irradiation caused a G2 arrest, increasing the G2/M fragment of the cells. Apoptosis by gamma-irradiation was apparent both by DNA-electrophoresis and PARP-1 cleavage. The combination of olomoucine with irradiation caused an increased G2 arrest and decreased cell survival and DNA synthesis in the non-apoptotic fraction of the remaining cells. Irradiation, as well as olomoucine and the combination of both, induced apoptosis. It seems that olomoucine delays the apoptotic process and inhibits DNA fragmentation, but it decreased survival, cell cycle progression and proliferation of irradiated cells. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved