PT  - JOURNAL ARTICLE
AU  - BOUTIS, ANASTASIOS
AU  - PAPAZISIS, KONSTANTINOS
AU  - PISTEVOU-GOMPAKI, KYRIAKI
AU  - LAMBROPOULOS, ALEXANDROS
AU  - SOFRONIADIS, IOANNIS
AU  - PAPAGEORGIOU, ATHANASIOS
AU  - DESTOUNI, EVANGELIA
AU  - KORTSARIS, ALEXANDROS
TI  - Cyclin-dependent Kinase (CDK) Inhibitor Olomoucine Enhances γ-Irradiation-induced Apoptosis and Cell Cycle Arrest in Raji Cells
DP  - 2006 Sep 01
TA  - Anticancer Research
PG  - 3493--3498
VI  - 26
IP  - 5A
4099  - http://ar.iiarjournals.org/content/26/5A/3493.short
4100  - http://ar.iiarjournals.org/content/26/5A/3493.full
SO  - Anticancer Res2006 Sep 01; 26
AB  - Gamma-irradiation leads to apoptosis and cell cycle arrest in eukaryotic cells. Olomoucine is a novel purine analog acting as a cyclin-dependent kinase inhibitor. The effects of olomoucine in gamma-irradiation mediated cell growth inhibition and apoptosis were studied in the Raji cell line (Burkitt's lymphoma). Gamma-irradiation caused a G2 arrest, increasing the G2/M fragment of the cells. Apoptosis by gamma-irradiation was apparent both by DNA-electrophoresis and PARP-1 cleavage. The combination of olomoucine with irradiation caused an increased G2 arrest and decreased cell survival and DNA synthesis in the non-apoptotic fraction of the remaining cells. Irradiation, as well as olomoucine and the combination of both, induced apoptosis. It seems that olomoucine delays the apoptotic process and inhibits DNA fragmentation, but it decreased survival, cell cycle progression and proliferation of irradiated cells. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved