RT Journal Article
SR Electronic
T1 Cytokine Genes Single Nucleotide Polymorphism (SNP) Screening Analyses in Canine Malignant Histiocytosis
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3417
OP 3420
VO 26
IS 5A
A1 SOLLER, JAN THIES
A1 ESCOBAR, HUGO MURUA
A1 JANSSEN, MIRIAM
A1 FORK, MELANI
A1 BULLERDIEK, JÖRN
A1 NOLTE, INGO
YR 2006
UL http://ar.iiarjournals.org/content/26/5A/3417.abstract
AB In humans, malignant histiocytosis is a tumour-like disease characterised by increasing proliferation of macrophages and reinforced degradation of erythrocytes. High progression of this disease leads to an unfavourable prognosis for the patients, most of them children up to the age of three years. Histological and cytological findings have proposed an important role of aberrant expression of cytokines in histiocytosis. Due to the fact that Bernese Mountain Dogs (BMD) show a predisposition for spontaneously developing malignant histiocytosis, these dogs could possibly be used as a genetic model organism to elucidate the mechanisms of human malignant histiocytosis. Canine cytokine cDNA transcripts of TNFα, Interleukin-1-α (IL-1α) and Interleukin-1-beta (IL-1β) were screened for single nucleotide polymorphisms (SNPs). SNP screening in canine cytokine transcripts for malignant histiocytosis has not been carried out before. Total RNA was isolated from tissue samples from lung, spleen, testis and skin of 17 different dogs (fifteen BMDs, one Collie and one West Highland Terrier). The corresponding cytokine cDNAs were amplified, sequenced and then screened for SNPs. The resulting effects on the protein sequence were analysed. Several BMDs and the West Highland Terrier showed SNPs in the coding sequences which led to missense mutations within the protein sequences of TNFα, IL1α and IL1β. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved