RT Journal Article
SR Electronic
T1 Mitochondrial DNA Mutations and 8-hydroxy-2′-deoxyguanosine Content in Japanese Patients with Urinary Bladder and Renal Cancers
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3403
OP 3408
VO 26
IS 5A
A1 TAKASHI WADA
A1 NOZOMU TANJI
A1 AKIRA OZAWA
A1 JIANBO WANG
A1 KENJI SHIMAMOTO
A1 KENSHI SAKAYAMA
A1 MASAYOSHI YOKOYAMA
YR 2006
UL http://ar.iiarjournals.org/content/26/5A/3403.abstract
AB Background: Several recent studies have demonstrated the presence of mitochondrial DNA (mtDNA) mutations in various human cancers. The origin of these mutations may be attributable to oxidative damage from reactive oxygen species (ROS). In order to investigate the relationship between mtDNA mutations and ROS in human cancers, urinary bladder and renal cancers were examined for mutations in the displacement-loop (D-loop) region of mtDNA and for 8-hydroxy-2′-deoxyguanosine (8-OHdG) content. Materials and Methods: The D-loop region of mtDNA of Japanese patients with urinary bladder or renal cancers was examined by direct sequencing. The level of 8-OHdG was measured in the patients who had undergone radical cystectomy or nephrectomy from excised specimens. Results: Somatic mutations in the D-loop region were detected in 7 (23%) out of 31 patients with bladder cancer and 3 (14%) out of 21 patients with renal cancer. The most frequent mutations were in the poly(C) mononucleotide repeat located at positions 303 to 309. The levels of 8-OHdG in cancer tissues were significantly higher than in the neighboring non-cancerous tissues, but many of the cancers with an elevated 8-OHdG level did not display D-loop mutations. Conclusion: These results suggest that the D-loop region of mtDNA might have a genetic instability in cancer tissues independently from the 8-OHdG level. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved