RT Journal Article
SR Electronic
T1 Antitumor Effect of Galectin-9 on Cell Proliferation and Tumor Growth of Human Duodenal Adenocarcinoma Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3769
OP 3777
DO 10.21873/anticanres.16562
VO 43
IS 8
A1 KONO, TOSHIAKI
A1 FUJIHARA, SHINTARO
A1 OURA, KYOKO
A1 IWAMA, HISAKAZU
A1 FUJITA, KOJI
A1 FUJITA, NAOKI
A1 TAKUMA, KEI
A1 ONO, MASAHIRO
A1 NAMIMA, DAISUKE
A1 YAMANA, HIROKI
A1 CHIYO, TAIGA
A1 KOBAYASHI, KIYOYUKI
A1 KAMADA, HIDEKI
A1 KOBARA, HIDEKI
A1 ONO, MASAHUMI
A1 NIKI, TOSHIRO
A1 HIRASHIMA, MITSUOMI
A1 MASAKI, TSUTOMU
YR 2023
UL http://ar.iiarjournals.org/content/43/8/3769.abstract
AB Background/Aim: Galectin-9 (Gal-9) induces tumor cell apoptosis in lymphoma and other malignant cell types. Duodenal adenocarcinoma is a rare malignancy, and there are insufficient data to determine a standard therapeutic approach. Here, we investigated the antitumor effect of Gal-9 in HuTu-80 duodenal adenocarcinoma cells. Materials and Methods: Cell proliferation was examined in HuTu-80 cells using a Cell Counting Kit-8 assay. Cell cycle analysis, apoptosis array, and microRNA expression analysis were performed to identify the effect of Gal-9 on HuTu-80 cells. The antitumor effect of Gal-9 was also examined using xenograft mouse models. Results: Gal-9 suppressed the proliferation of HuTu-80 via blockade of the G0 to G1 cell cycle transition. This blockade was accompanied by a strong decrease in cyclin D1 and phosphorylated Rb, suggesting a G1 arrest. Additionally, Gal-9 induced apoptosis, and the expression of cleaved caspase-3 was increased in Gal-9-treated HuTu-80 cells according to the apoptosis array. MiRNA microarrays revealed that Gal-9 altered the expression of miRNAs in HuTu-80 cells. Conclusion: These data demonstrate the therapeutic potential of Gal-9 and provide molecular mechanistic insights into its antitumor effect in HuTu-80 cells.