TY - JOUR T1 - Establishment and Characterization of a New Cell Line, FPS-1, Derived from Human Undifferentiated Pleomorphic Sarcoma, Overexpressing Epidermal Growth Factor Receptor and Cyclooxygenase-2 JF - Anticancer Research JO - Anticancer Res SP - 3393 LP - 3401 VL - 26 IS - 5A AU - MICHIYUKI HAKOZAKI AU - HIROSHI HOJO AU - MICHIKO SATO AU - TAKAHIRO TAJINO AU - HITOSHI YAMADA AU - SHINICHI KIKUCHI AU - MASAFUMI ABE Y1 - 2006/09/01 UR - http://ar.iiarjournals.org/content/26/5A/3393.abstract N2 - Background: Undifferentiated pleomorphic sarcoma (UPS) is among the most common soft tissue sarcomas in adults. In order to improve its aggressive course or prognosis and establish new therapeutic methods, molecular genetic and biological characterizations of UPS are required. Materials and Methods: A new human UPS cell line (FPS-1) was established from UPS of the upper arm of a 79-year-old man. The cell line has been maintained for over 14 months with more than 60 passages. FPS-1 cells were characterized using molecular biological methods. Results: FPS-1 cells showed the same morphological and immunophenotypical characteristics as the primary tumor. Cytogenetic and molecular analyses revealed a nonsense mutation in exon 6 of the p53 gene. Epidermal growth factor receptor (EGFR) and cyclooxygenase-2 (COX-2) were expressed in FPS-1 cells. Conclusion: FPS-1 cells might be useful for investigating biological behavior and developing new molecular targeting antitumor drugs for UPS with EGFR or COX-2 expression. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -