TY - JOUR T1 - High Energy Shock Waves (HESW) for Sonodynamic Therapy: Effects on HT-29 Human Colon Cancer Cells JF - Anticancer Research JO - Anticancer Res SP - 3337 LP - 3342 VL - 26 IS - 5A AU - ROBERTO CANAPARO AU - LOREDANA SERPE AU - MARIA GRAZIELLA CATALANO AU - ORNELLA BOSCO AU - GIAN PAOLO ZARA AU - LAURA BERTA AU - ROBERTO FRAIRIA Y1 - 2006/09/01 UR - http://ar.iiarjournals.org/content/26/5A/3337.abstract N2 - Background: Whether high energy shock waves (HESW), generated by a piezoelectric generator, were able to activate a sonosensitizer, 5-aminolevulinic acid (ALA) and induce inhibition of cell growth in HT-29 human colorectal cancer cells was investigated. Materials and Methods: Cell survival and cell death pathways were investigated by cell growth curves, flow cytometry analysis and ELISA nucleosome evaluation. HT-29 cells were exposed to ALA and different HESW treatments: E1 (energy flux density = 0.22 mJ/mm2; 500 and 1000 shots) and E2 (energy flux density = 0.88 mJ/mm2; 500 and 1000 shots). Results: A significant reduction of HT-29 cell growth with respect to untreated cells was observed only after treatment with ALA and HESW E2, 500 shots. In particular, HESW E2, 500 shots, was able to induce an arrest of HT-29 cells exposed to ALA in the G0/G1-phase of the cell cycle. Conclusion: HESW is proposed for the sonodynamic treatment of cancer cells. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -