TY - JOUR T1 - Relationship Between EGFR Expression in Oral Cancer Cell Lines and Cetuximab Antibody-dependent Cell-mediated Cytotoxicity JF - Anticancer Research JO - Anticancer Res SP - 1275 LP - 1282 DO - 10.21873/anticanres.13238 VL - 39 IS - 3 AU - HIROSHI NAKAMURA AU - SHIGEHIRO TAMAKI AU - TAKAHIRO YAGYUU AU - NOBUHIRO YAMAKAWA AU - KATSUHIKO HATAKE AU - TADAAKI KIRITA Y1 - 2019/03/01 UR - http://ar.iiarjournals.org/content/39/3/1275.abstract N2 - Background/Aim: Cetuximab treatment targets the epidermal growth factor receptor (EGFR) overexpressed in oral cancer. This study aimed to investigate the anti-tumour activity of cetuximab against oral cancer cell lines with respect to antibody-dependent cell-mediated cytotoxicity (ADCC), and determine the correlation between ADCC and EGFR expression. Materials and Methods: EGFR expression in oral cancer cells was measured by quantitative flow cytometric analysis and immunohistochemistry. ADCC activity was measured by 4-h calcein release assays. Results: Cetuximab-mediated ADCC against oral cancer cells was detectable at a concentration of 0.1 μg/ml. A high correlation was observed between the number of EGFR molecules on the surface of oral cancer cells and ADCC (correlation coefficient: 0.847; p=0.032). Conclusion: ADCC is an important mechanism underlying the therapeutic effect of cetuximab, and EGFR expression in tumour cells might serve as a predictive marker to evaluate the effect of cetuximab treatment. ER -