RT Journal Article
SR Electronic
T1 <em>P4HA1</em> Promotes Cell Migration and Colonization in Hypopharyngeal Squamous Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2571
OP 2582
DO 10.21873/anticanres.16424
VO 43
IS 6
A1 SATO, KANAME
A1 SANO, DAISUKE
A1 TAKAHASHI, HIDEAKI
A1 KUWAHARA, TATSU
A1 AIZAWA, YOSHIHIRO
A1 AOYAMA, JUN
A1 NOJIMA, YUSUKE
A1 HATANO, TAKASHI
A1 ARAI, YASUHIRO
A1 NISHIMURA, GOSHI
A1 HATAKEYAMA, HIROMITSU
A1 ORIDATE, NOBUHIKO
YR 2023
UL http://ar.iiarjournals.org/content/43/6/2571.abstract
AB Background/Aim: This study aimed to identify key molecules associated with the survival of patients with hypopharyngeal squamous cell carcinoma (HpSCC) by combining in silico and in vitro analyses. Materials and Methods: Differentially expressed genes (DEGs) were screened using the Gene Expression Omnibus database. For DEGs, we performed functional enrichment and protein–protein interaction network analyses to identify potential biological functions and hub genes. Functional analysis of HpSCC cell lines verified the critical roles of the hub genes. Results: DEGs were associated with the extracellular matrix. Among the hub genes, high expression of prolyl 4-hydroxylase subunit alpha 1 (P4HA1) was significantly associated with shorter survival. In addition, P4HA1 knockdown inhibited cell migration and colonization. Suppression of cell proliferation was demonstrated using P4HA1-selective inhibitors. Conclusion: P4HA1 may be a useful therapeutic target for the treatment of HpSCC.