RT Journal Article
SR Electronic
T1 Tumor DNA in Peritoneal Lavage as a Novel Biomarker for Predicting Peritoneal Recurrence in Patients With Gastric Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2069
OP 2076
DO 10.21873/anticanres.16367
VO 43
IS 5
A1 YUKAWA, NORIO
A1 YAMADA, TAKESHI
A1 AOYAMA, TORU
A1 WOO, TEKKAN
A1 UEDA, KOJI
A1 MASTUDA, AKIHISA
A1 HARA, KENTARO
A1 KAZAMA, KEISUKE
A1 TAMAGAWA, HIROSHI
A1 SATO, TSUTOMU
A1 OSHIMA, TAKASHI
A1 SUZUKI, AKIHIRO
A1 ABURATANI, HIROYUKI
A1 ISHIKAWA, SHUMPEI
A1 SAITO, AYA
A1 MASUDA, MUNETAKA
A1 YOSHIDA, HIROSHI
A1 RINO, YASUSHI
YR 2023
UL http://ar.iiarjournals.org/content/43/5/2069.abstract
AB Background: Peritoneal lavage cytology positivity (CY1) has been identified as a prognostic factor for distant metastases that is equivalent to peritoneal dissemination in Japan. Peritoneal lavage cytology is usually diagnosed by microscopic findings; a diagnostic procedure using a liquid biopsy (LB) technique has not yet been established. Patients and Methods: We evaluated the feasibility of a LB approach using peritoneal lavage samples from 15 patients with gastric cancer. Samples were collected from both the Douglas pouch and the left subdiaphragmatic area, and cell-free DNA was extracted for analysis of TP53 mutations using droplet digital polymerase chain reaction. Results: All 10 patients with CY1 had positive cytology for the left subdiaphragmatic specimen. However, only six out of the 10 patients had positive cytology for their Douglas pouch specimens, and these six patients had peritoneal tumor DNA (ptDNA) in these specimens. In all five patients with CY0, ptDNA was not detected. The overall survival was significantly shorter in the ptDNA-positive group than in the ptDNA-negative group. The survival of the group with a high amount of DNA from free intraperitoneal cells (ficDNA) was significantly worse than that of those with a low amount. In contrast, the group with a high amount of DNA from peritoneal cell-free DNA (pcfDNA) had significantly better survival than the group with a low amount. Conclusion: LB cytology showed equivalent utility to that of conventional microscopic examinations regarding its diagnostic ability. Therefore ptDNA, pcfDNA and ifcDNA are expected to be useful as prognostic factors.