PT  - JOURNAL ARTICLE
AU  - YUKAWA, NORIO
AU  - YAMADA, TAKESHI
AU  - AOYAMA, TORU
AU  - WOO, TEKKAN
AU  - UEDA, KOJI
AU  - MASTUDA, AKIHISA
AU  - HARA, KENTARO
AU  - KAZAMA, KEISUKE
AU  - TAMAGAWA, HIROSHI
AU  - SATO, TSUTOMU
AU  - OSHIMA, TAKASHI
AU  - SUZUKI, AKIHIRO
AU  - ABURATANI, HIROYUKI
AU  - ISHIKAWA, SHUMPEI
AU  - SAITO, AYA
AU  - MASUDA, MUNETAKA
AU  - YOSHIDA, HIROSHI
AU  - RINO, YASUSHI
TI  - Tumor DNA in Peritoneal Lavage as a Novel Biomarker for Predicting Peritoneal Recurrence in Patients With Gastric Cancer
AID  - 10.21873/anticanres.16367
DP  - 2023 May 01
TA  - Anticancer Research
PG  - 2069--2076
VI  - 43
IP  - 5
4099  - http://ar.iiarjournals.org/content/43/5/2069.short
4100  - http://ar.iiarjournals.org/content/43/5/2069.full
SO  - Anticancer Res2023 May 01; 43
AB  - Background: Peritoneal lavage cytology positivity (CY1) has been identified as a prognostic factor for distant metastases that is equivalent to peritoneal dissemination in Japan. Peritoneal lavage cytology is usually diagnosed by microscopic findings; a diagnostic procedure using a liquid biopsy (LB) technique has not yet been established. Patients and Methods: We evaluated the feasibility of a LB approach using peritoneal lavage samples from 15 patients with gastric cancer. Samples were collected from both the Douglas pouch and the left subdiaphragmatic area, and cell-free DNA was extracted for analysis of TP53 mutations using droplet digital polymerase chain reaction. Results: All 10 patients with CY1 had positive cytology for the left subdiaphragmatic specimen. However, only six out of the 10 patients had positive cytology for their Douglas pouch specimens, and these six patients had peritoneal tumor DNA (ptDNA) in these specimens. In all five patients with CY0, ptDNA was not detected. The overall survival was significantly shorter in the ptDNA-positive group than in the ptDNA-negative group. The survival of the group with a high amount of DNA from free intraperitoneal cells (ficDNA) was significantly worse than that of those with a low amount. In contrast, the group with a high amount of DNA from peritoneal cell-free DNA (pcfDNA) had significantly better survival than the group with a low amount. Conclusion: LB cytology showed equivalent utility to that of conventional microscopic examinations regarding its diagnostic ability. Therefore ptDNA, pcfDNA and ifcDNA are expected to be useful as prognostic factors.