RT Journal Article SR Electronic T1 The Application of Static Magnetic Stimulation Reduces Survival of SH-SY5Y Neuroblastoma Cells JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1427 OP 1436 DO 10.21873/anticanres.16291 VO 43 IS 4 A1 HELOUISE RICHARDT MEDEIROS A1 JOSÉ ANTÔNIO FAGUNDES ASSUMPÇÃO A1 DIRSON JOÃO STEIN A1 EDUARDO CREMONESE FILIPPI-CHIELA A1 FELIPE FREGNI A1 WOLNEI CAUMO A1 PAULO ROBERTO STEFANI SANCHES A1 IRACI L.S. TORRES YR 2023 UL http://ar.iiarjournals.org/content/43/4/1427.abstract AB Background/Aim: Central nervous system cancer is still a major public health issue. The effectiveness of treatments is limited and varies depending on the severity of disease. Therefore, there is a demand for the development of novel therapies. Static magnetic stimulation (SMS) emerges as a new therapeutic option. The aim of this study was to evaluate the SMS effects on neuroblastoma cells in culture. Materials and Methods: SH-SY5Y neuroblastoma cells were exposed to 0.3T SMS for 6, 12, 24, 36, 72 h, and 6 days. Cell viability (MTT), cell death (annexin-V/PI staining) and cell cycle (DNA content), cell proliferation (CFSE), autophagy (acridine orange), and total mitochondrial mass (MitoTracker™ Red) were analyzed to establish the cellular response to SMS. Results: The viability of SH-SY5Y cells was reduced after exposure to SMS for 24 h and 6 days (p<0.05), without differences for the other times (p>0.05); however, this effect was not related to cell death or cell cycle arrest (p>0.05). In contrast, the viability of human malignant melanoma (HMV-II) cells, used as a tumoral control, was not affected. In addition, stimulated SH-SY5Y cells presented a decrease in mitochondrial mass at both exposure times and a reduction in autophagy and cell proliferation after 6 days (p<0.05). Conclusion: SMS application appears to be a promising adjuvant therapy for the treatment of neuroblastoma since it decreases the survival of SH-SY5Y neuroblastoma cells.