PT  - JOURNAL ARTICLE
AU  - PAULINA PRZYCHODZEN
AU  - ROKSANA WYSZKOWSKA
AU  - MONIKA GORZYNIK-DEBICKA
AU  - TOMASZ KOSTRZEWA
AU  - ALICJA KUBAN-JANKOWSKA
AU  - MAGDALENA GORSKA-PONIKOWSKA
TI  - Anticancer Potential of Oleuropein, the Polyphenol of Olive Oil, With 2-Methoxyestradiol, Separately or in Combination, in Human Osteosarcoma Cells
AID  - 10.21873/anticanres.13234
DP  - 2019 Mar 01
TA  - Anticancer Research
PG  - 1243--1251
VI  - 39
IP  - 3
4099  - http://ar.iiarjournals.org/content/39/3/1243.short
4100  - http://ar.iiarjournals.org/content/39/3/1243.full
SO  - Anticancer Res2019 Mar 01; 39
AB  - Background/Aim: Oleuropein belongs to the potent polyphenols of olive oil. Notably, it is considered as a potentially active anticancer agent. Herein, the anticancer efficiency of oleuropein, when used separately and in combination with the chemotherapeutic agent, 2-methoxyestradiol (2-ME), was investigated in highly metastatic osteosarcoma (OS) cells. Materials and Methods: Human OS cells (143B OS cell line) were incubated with oleuropein and 2-ME, alone or in combination. Cell viability was determined by the MTT assay. Cell migration assays were used in order to determine the anti-migratory potential of the compounds, while their impact on autophagy was evaluated via the LC3-antibody-based detection assay. The interaction between oleuropein and 2-ME was determined via the CalcuSyn software. Results: Both anti-migratory and anti-proliferative effects of oleuropein were demonstrated on human OS cells. Anticancer effects of oleuropein were significantly enhanced after 2-ME addition. Treatment of 143B OS cell with oleuropein, alone or in combination with 2-ME resulted in induction of autophagy. Conclusion: The obtained data suggest an anticancer effect of oleuropein, alone and in combination with 2-ME, on highly metastatic 143B OS cells. Notably, a synergism between oleuropein and 2-ME towards 143B OS cells was detected. The exact mechanism of this synergism needs to be further investigated; nonetheless, induction of nitro-oxidative stress and/or induction of autophagy are suggested.