PT - JOURNAL ARTICLE AU - BYUN, JUNGSUP AU - PARK, NORA JEE-YOUNG AU - YOON, GHILSUK AU - KANG, MIN KYU AU - KIM, HYE JIN AU - PARK, SOO YEUN AU - PARK, JUN SEOK AU - CHOI, GYU SEOG AU - BAEK, JIN HO AU - KIM, JONG GWANG AU - SEO, AN NA TI - <em>PIK3CA</em> Mutations as a Prognostic Factor in Patients With Residual Rectal Cancer After Neoadjuvant Chemoradiotherapy AID - 10.21873/anticanres.16300 DP - 2023 Apr 01 TA - Anticancer Research PG - 1513--1520 VI - 43 IP - 4 4099 - http://ar.iiarjournals.org/content/43/4/1513.short 4100 - http://ar.iiarjournals.org/content/43/4/1513.full SO - Anticancer Res2023 Apr 01; 43 AB - Background/Aim: PIK3CA mediates various cellular processes, such as transformation, tumor initiation and proliferation, and resistance to apoptosis. This study was conducted to identify the clinical significance and prognostic effect of PIK3CA mutations in patients with residual rectal cancer who underwent surgery after neoadjuvant chemoradiotherapy (NACRT). Patients and Methods: Formalin-fixed and paraffin-embedded surgical specimens were collected from 128 patients between January 2006 and December 2011 and analyzed using real-time polymerase chain reaction for hotspot mutations in exons 9 and 20 of the PIK3CA gene. Results: Of the 128 patients, 109 were analyzed and 19 were excluded because of poor DNA quality. Mutations in PIK3CA were identified in three patients (2.8%), all of which were detected in exon 20 of the PIK3CA gene. PIK3CA mutations significantly correlated with lymphatic invasion (p=0.016), lymph node metastasis (p=0.034), and higher pathological disease stage (p=0.040). By univariate analysis, patients with PIK3CA mutations were observed to have significantly shorter cancer-specific survival (CSS) (p=0.001) and disease-free survival (DFS) (p=0.006) than PIK3CA wild-type patients. However, PIK3CA mutations were not an independent prognostic factor for CSS (p=0.319) or DFS (p=0.219) in multivariate modeling. Conclusion: Our findings indicate that PIK3CA mutation plays a role in oncogenesis in rectal cancer and may be considered as a candidate therapeutic approach targeting the PIK3/Akt/mTOR pathway in patients with residual rectal cancer after NACRT.