RT Journal Article
SR Electronic
T1 Association Between a High Gene Expression Variant of Chymase and Increased Risk for Basal Cell Carcinoma
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5547
OP 5552
DO 10.21873/anticanres.16060
VO 42
IS 11
A1 SEVASTIANA CHARALAMPIDOU
A1 IPHIGENIA GINTONI
A1 DIMITRIS AVGOUSTIDIS
A1 VERONICA PAPAKOSTA
A1 DIMITRIOS VLACHAKIS
A1 STAVROS VASSILIOU
A1 CHRISTOS YAPIJAKIS
YR 2022
UL http://ar.iiarjournals.org/content/42/11/5547.abstract
AB Background/Aim: Previous studies have associated certain variations in genes encoding factors of renin-angiotensin system (RAS), indirectly leading to higher angiotensin II (AngII) levels, with greater risk for basal cell carcinoma (BCC) development. Chymase (CMA1) is the main regulator of the RAS-independent AngII generation pathway and numerous studies have shown its oncogenic potential in several cancer types including BCC. In this study, we investigated the possible association between BCC pathogenesis and the functional DNA polymorphism A1903G (rs1800875) that affects expression of the CMA1 gene. Patients and Methods: We genotyped 199 DNA samples, isolated from 100 BCC patients and 99 age, sex, and ethnicity-matched healthy controls for the CMA1 A1903G polymorphism. Genotyping was performed with PCR amplification, followed by MboI enzyme digestion and agarose gel electrophoresis of the resulted DNA fragments. Results: The variant G allele that possibly increases CMA1 gene expression was not detected at a significantly different frequency between the groups of BCC patients and healthy controls. However, the AG heterozygous genotype was significantly increased in BCC patients compared with controls (p<0.001). Conclusion: The high expression CMA1 G allele carriers have an increased risk for BCC and elevated levels of chymase in the skin may have a carcinogenic effect.