@article {ISHIGURO5315, author = {MASAKO ISHIGURO and TOMOKO FUKUSHIGE and HIROSHI IWASAKI and MAKOTO HAMASAKI}, title = {Establishment and Characterisation of Human Clear Cell Sarcoma and Malignant Melanoma Cell Lines}, volume = {42}, number = {11}, pages = {5315--5322}, year = {2022}, doi = {10.21873/anticanres.16038}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Clear cell sarcoma of soft tissue (CCSST) and conventional malignant melanoma (MM) are rare and aggressive tumours with similarities in morphology and the expression of melanocytic markers. Materials and Methods: We established two CCSST cell lines (FU-CCSST-1 and FU-CCSST-2) from soft tissues of the patella and supraclavicular. A MM cell line (FU-MM-1) was established from lymph node metastases of subungual malignant melanoma. Results: FU-CCSST-2 cells were transplantable to immunodeficient mice. Immunohistochemical studies demonstrated tumour cells were negative for cytokeratin AE1/AE3 and positive for S100 protein, HMB45, Melan-A, CD146 and SOX10 in all specimens. FU-CCSST-1 and FU-CCSST-2 harboured t(12;22)(q13;q12) translocations with expression of the EWSR1/ATF1 fusion gene. FU-MM-1 demonstrated loss of the short arm of chromosome 9 and harboured wild-type BRAF (codon 469 and 600) and NRAS (codon 12, 13 and 61). Conclusion: We report the establishment and characterisation of CCSST and MM cell lines that may have utility in the study of pathogenic mechanisms and development of novel therapeutic reagents.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/42/11/5315}, eprint = {https://ar.iiarjournals.org/content/42/11/5315.full.pdf}, journal = {Anticancer Research} }