RT Journal Article
SR Electronic
T1 HPV16 DNA in Histologically Confirmed Tumour-free Neck Lymph Nodes of Head and Neck Cancers
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 663
OP 670
VO 26
IS 1B
A1 HOFFMANN, MARKUS
A1 ORLAMÜNDER, ANTJE
A1 SUCHER, JAN
A1 GOTTSCHLICH, STEFAN
A1 GÖRÖGH, TIBOR
A1 FAZEL, ASITA
A1 AMBROSCH, PETRA
A1 RITTGEN, WERNER
A1 SCHWARZ, ELISABETH
A1 KAHN, TOMAS
YR 2006
UL http://ar.iiarjournals.org/content/26/1B/663.abstract
AB Background: Human papillomavirus (HPV) has been demonstrated in lymph node neck metastases (NM) of HPV-positive squamous cell carcinomas of the head and neck (HNSCC), underscoring the possible role of HPV for HNSCC progression. Reports on HPV infections in histopathologically tumour-free lymph-nodes of the SCC of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study. Materials and Methods: The presence of HPV-DNA in primary tumours (PT, n=45), NM (n=45) and histologically confirmed tumour-free neck lymph-nodes (LN, n=102) of HNSCC from 60 patients was analysed by PCR and Southern blot hybridisation. Results: A highly positive correlation of simultaneous HPV-DNA detection in PT and NM was demonstrated. In the case of HPV-positivity of PT and/or NM [24/60 cases (40%)], 11/24 (45.8%) LN contained HPV-DNA, as well. Accepting HPV demonstration as a marker for the presence of micro-metastasis, HPV analysis would result in an upstaging of the N category in 4 out of these 11 patients. Conclusion: Considering the high agreement of HPV-DNA detection in PT and simultaneous HPV-DNA demonstration in the draining NM corroborating the monoclonal character of the tumour cells, the HPV-DNA presence in LN seems to be indicative of micro-metastasis in these lymph nodes. Thus, HPV analysis might be another powerful tool for the definition of the N-status of HPV-positive HNSCC. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved