<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">HOFFMANN, MARKUS</style></author><author><style face="normal" font="default" size="100%">ORLAMÜNDER, ANTJE</style></author><author><style face="normal" font="default" size="100%">SUCHER, JAN</style></author><author><style face="normal" font="default" size="100%">GOTTSCHLICH, STEFAN</style></author><author><style face="normal" font="default" size="100%">GÖRÖGH, TIBOR</style></author><author><style face="normal" font="default" size="100%">FAZEL, ASITA</style></author><author><style face="normal" font="default" size="100%">AMBROSCH, PETRA</style></author><author><style face="normal" font="default" size="100%">RITTGEN, WERNER</style></author><author><style face="normal" font="default" size="100%">SCHWARZ, ELISABETH</style></author><author><style face="normal" font="default" size="100%">KAHN, TOMAS</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">HPV16 DNA in Histologically Confirmed Tumour-free Neck Lymph Nodes of Head and Neck Cancers</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006-01-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">663-670</style></pages><volume><style face="normal" font="default" size="100%">26</style></volume><issue><style face="normal" font="default" size="100%">1B</style></issue><abstract><style  face="normal" font="default" size="100%">Background: Human papillomavirus (HPV) has been demonstrated in lymph node neck metastases (NM) of HPV-positive squamous cell carcinomas of the head and neck (HNSCC), underscoring the possible role of HPV for HNSCC progression. Reports on HPV infections in histopathologically tumour-free lymph-nodes of the SCC of the uterine cervix developing higher rates of lymph-node metastases and recurrences later in the survey of the patients was the starting point of the present study. Materials and Methods: The presence of HPV-DNA in primary tumours (PT, n=45), NM (n=45) and histologically confirmed tumour-free neck lymph-nodes (LN, n=102) of HNSCC from 60 patients was analysed by PCR and Southern blot hybridisation. Results: A highly positive correlation of simultaneous HPV-DNA detection in PT and NM was demonstrated. In the case of HPV-positivity of PT and/or NM [24/60 cases (40%)], 11/24 (45.8%) LN contained HPV-DNA, as well. Accepting HPV demonstration as a marker for the presence of micro-metastasis, HPV analysis would result in an upstaging of the N category in 4 out of these 11 patients. Conclusion: Considering the high agreement of HPV-DNA detection in PT and simultaneous HPV-DNA demonstration in the draining NM corroborating the monoclonal character of the tumour cells, the HPV-DNA presence in LN seems to be indicative of micro-metastasis in these lymph nodes. Thus, HPV analysis might be another powerful tool for the definition of the N-status of HPV-positive HNSCC. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved</style></abstract></record></records></xml>