RT Journal Article
SR Electronic
T1 Targeted and Efficient Delivery of siRNA Using Tunable Polymeric Hybrid Micelles for Tumor Therapy
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1169
OP 1178
DO 10.21873/anticanres.13226
VO 39
IS 3
A1 FEI HAO
A1 SHIYAN DONG
A1 CHUNMIAO YANG
A1 ZIWEI LI
A1 ZIYUAN CHENG
A1 LIHUANG ZHONG
A1 LIRONG TENG
A1 QINGFAN MENG
A1 JIAHUI LU
A1 FAN WU
A1 JING XIE
A1 LESHENG TENG
A1 ROBERT J. LEE
YR 2019
UL http://ar.iiarjournals.org/content/39/3/1169.abstract
AB Background/Aim: Effective and targeted delivery of siRNA to tumor cells is a prerequisite to achieving their therapeutic effects. Survivin is up-regulated in tumor cells and is associated with resistance to therapy. Therefore, siRNA-mediated silencing of survivin is a potential therapeutic strategy for cancer. The aim of the study was to examine whether polymeric hybrid micelles can be used to effectively deliver siRNAs into cells. Materials and Methods: First, linoleic acid (LA) was conjugated to polyethylenimine (PEI) and methoxy-polyethyleneglycol (mPEG) and two amphiphilic polymers (PEI-LA and mPEG-LA) were obtained. Polymeric hybrid micelle (PHM) was then prepared and characterized by self-assembly of PEI-LA and mPEG-LA at different percentages of the two amphiphilic polymers. A PHM/siRNA complex with optimized composition and good biocompatibility was then prepared and its cellular uptake, biodistribution, and antitumor effects were investigated. Results: Survivin siRNA was efficiently delivered to the cells. It reduced survivin protein expression and greatly suppressed tumor growth. Moreover, siRNA loaded in PHM gathered in a solid tumor in mice and achieved an improved anticancer effect compared to naked siRNA. Conclusion: PHM is a promising and safe vehicle for siRNA delivery and may find utility in cancer therapy.