RT Journal Article
SR Electronic
T1 Standardized Methods Using EUS-guided Fine-needle Biopsy and a Minimal Medium Creates Three Pancreatic Cancer Organoids
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 4103
OP 4109
DO 10.21873/anticanres.15908
VO 42
IS 8
A1 YUSUKE ISHIDA
A1 TOSHIYUKI TSUNODA
A1 YOSHIHIRO HAMADA
A1 NAOAKI TSUCHIYA
A1 TAKEHIKO KOGA
A1 TAKANORI KITAGUCHI
A1 KEISUKE MATSUMOTO
A1 SHINJI MATSUMOTO
A1 TAKAHIDE SASAKI
A1 RYO NAKASHIMA
A1 FUMINORI ISHII
A1 MASATOSHI KAJIWARA
A1 SENJI SHIRASAWA
A1 SUGURU HASEGAWA
A1 FUMIHITO HIRAI
YR 2022
UL http://ar.iiarjournals.org/content/42/8/4103.abstract
AB Background/Aim: Recently, endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) has been conducted for diagnosing pancreatic ductal adenocarcinoma (PDAC), after which obtained samples were used in organoid cultures. However, no standardized method for PDAC organoid cultures exists. Therefore, to standardize or simplify sample collection and culture methods for PDAC organoids, we performed a floating culture using non-minced specimens obtained by EUS-FNB in a minimal medium, lacking growth factors or inhibitors for pancreatic organoids. Patients and Methods: A total of 38 patients with clinically diagnosed PDAC were enrolled in the study. First, EUS-FNB was conducted using a 22- or 25-gauge biopsy needle. Then, a surplus of samples was collected for organoid formation after rapid on-site cytological evaluations of sample adequacy. Subsequently, the established organoids were compared with clinical data and pathological diagnosis, following periodic observations and evaluations for morphology. Results: PDAC organoids were successfully created in 24 of the 38 cases (63.2%), including four cases with pathologically inconclusive EUS-FNB results. Afterward, PDAC organoid morphology was classified into ductal, dormant, and adhesive small cluster (ASC) types. Although the ductal and ASC types were seen separately, they were also seen together in other cases, which we named “mixed type”. Conclusion: We propose a feasible and straightforward method for establishing organoids, especially for diagnosing PDAC, particularly when the result of EUS-FNB is pathologically inconclusive. Furthermore, PDAC organoids are morphologically classified into three types reported for the first time.