PT  - JOURNAL ARTICLE
AU  - ISHIDA, YUSUKE
AU  - TSUNODA, TOSHIYUKI
AU  - HAMADA, YOSHIHIRO
AU  - TSUCHIYA, NAOAKI
AU  - KOGA, TAKEHIKO
AU  - KITAGUCHI, TAKANORI
AU  - MATSUMOTO, KEISUKE
AU  - MATSUMOTO, SHINJI
AU  - SASAKI, TAKAHIDE
AU  - NAKASHIMA, RYO
AU  - ISHII, FUMINORI
AU  - KAJIWARA, MASATOSHI
AU  - SHIRASAWA, SENJI
AU  - HASEGAWA, SUGURU
AU  - HIRAI, FUMIHITO
TI  - Standardized Methods Using EUS-guided Fine-needle Biopsy and a Minimal Medium Creates Three Pancreatic Cancer Organoids
AID  - 10.21873/anticanres.15908
DP  - 2022 Aug 01
TA  - Anticancer Research
PG  - 4103--4109
VI  - 42
IP  - 8
4099  - http://ar.iiarjournals.org/content/42/8/4103.short
4100  - http://ar.iiarjournals.org/content/42/8/4103.full
SO  - Anticancer Res2022 Aug 01; 42
AB  - Background/Aim: Recently, endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) has been conducted for diagnosing pancreatic ductal adenocarcinoma (PDAC), after which obtained samples were used in organoid cultures. However, no standardized method for PDAC organoid cultures exists. Therefore, to standardize or simplify sample collection and culture methods for PDAC organoids, we performed a floating culture using non-minced specimens obtained by EUS-FNB in a minimal medium, lacking growth factors or inhibitors for pancreatic organoids. Patients and Methods: A total of 38 patients with clinically diagnosed PDAC were enrolled in the study. First, EUS-FNB was conducted using a 22- or 25-gauge biopsy needle. Then, a surplus of samples was collected for organoid formation after rapid on-site cytological evaluations of sample adequacy. Subsequently, the established organoids were compared with clinical data and pathological diagnosis, following periodic observations and evaluations for morphology. Results: PDAC organoids were successfully created in 24 of the 38 cases (63.2%), including four cases with pathologically inconclusive EUS-FNB results. Afterward, PDAC organoid morphology was classified into ductal, dormant, and adhesive small cluster (ASC) types. Although the ductal and ASC types were seen separately, they were also seen together in other cases, which we named “mixed type”. Conclusion: We propose a feasible and straightforward method for establishing organoids, especially for diagnosing PDAC, particularly when the result of EUS-FNB is pathologically inconclusive. Furthermore, PDAC organoids are morphologically classified into three types reported for the first time.