PT - JOURNAL ARTICLE AU - SUGURU OGATA AU - TOMOYA SUDOU AU - FUMIHIKO FUJITA AU - TAKEFUMI YOSHIDA AU - KENICHI KOUSHI AU - TAKASHI NOGUCHI AU - JUNYA FUKUDA AU - NANAE OGATA AU - YOSHITAKA NOBUKUNI AU - YOSHITO AKAGI TI - Experimental Exploration for Genes Related to Susceptibility and Resistance to Irinotecan AID - 10.21873/anticanres.15897 DP - 2022 Aug 01 TA - Anticancer Research PG - 4011--4016 VI - 42 IP - 8 4099 - http://ar.iiarjournals.org/content/42/8/4011.short 4100 - http://ar.iiarjournals.org/content/42/8/4011.full SO - Anticancer Res2022 Aug 01; 42 AB - Background/Aim: Anticancer drug resistance is an important issue in cancer treatment. Multiple genes are thought to be involved in resistance to anticancer drugs; however, this is still not fully understood. This study aimed to identify the genes involved in irinotecan resistance and their functional characteristics. Materials and Methods: Gene trap insertion mutant Chinese hamster ovary (CHO) cells were used in the experiments, and next-generation sequencing, gene-ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to evaluate the biological functions of differentially expressed genes (DEGs). Results: In total, 2,134 DEGs were identified, including 1,216 up-regulated and 918 down-regulated genes. In KEGG pathways, microRNAs in cancer were significantly associated with up-regulated DEGs, while spliceosome and p53 signaling pathways were significantly associated with down-regulated DEGs. The pathway analysis identified several genes that might be involved in irinotecan resistance. Conclusion: Using CHO cells, the genes involved in irinotecan resistance and functional characteristics were predicted. These results provide new clues for predicting irinotecan resistance.