PT - JOURNAL ARTICLE AU - KOJI WAKAME AU - KEISUKE SATO AU - MASAFUMI KASAI AU - EMI KIKUCHI AU - KIHIRO SHIMIZU AU - AKO KUDO AU - KEN-ICHI KOMATSU AU - AKIFUMI NAKATA TI - Oral Administration of Mulberry (<em>Morus alba</em> L.) Leaf Powder Prevents the Development of Hepatocellular Carcinoma in Stelic Animal Model (STAM) Mice AID - 10.21873/anticanres.15902 DP - 2022 Aug 01 TA - Anticancer Research PG - 4055--4062 VI - 42 IP - 8 4099 - http://ar.iiarjournals.org/content/42/8/4055.short 4100 - http://ar.iiarjournals.org/content/42/8/4055.full SO - Anticancer Res2022 Aug 01; 42 AB - Background/Aim: We examined the inhibitory effect of mulberry leaf (ML) (Morus alba L.) administration on the development of hepatocellular carcinoma (HCC) in stelic animal model (STAM) mice. This STAM mouse model of nonalcoholic steatohepatitis (NASH) closely resembles the progression from NASH to HCC in human clinical practice. Materials and Methods: Streptozotocin (STZ, 200 μg) was administered to C57L/6J mice that were fed a high-fat diet (HFD; STAM mice) with 1% ML ad libitum. After sacrificing, the liver and blood were collected. Biochemical tests of plasma and histologic examination of the liver were performed. Results: Pathologic examination of all (6/6) liver samples of the STAM mice showed HCC. On the contrary, in STAM mice that received ML, fat deposition and adenoma were observed in 6/6 and 2/6 of the liver samples, respectively, but there was no HCC. Conclusion: Administration of ML in STAM mice inhibited the progression from nonalcoholic hepatitis (NASH) to HCC. ML may be effective in preventing the development of HCC.