%0 Journal Article %A Y.M. CHONG %A S.L.J. WILLIAMS %A A. ELKAK %A A.K. SHARMA %A K. MOKBEL %T Insulin-like Growth Factor 1 (IGF-1) and its Receptor mRNA Levels in Breast Cancer and Adjacent Non-neoplastic Tissue %D 2006 %J Anticancer Research %P 167-173 %V 26 %N 1A %X Previous studies investigating the insulin-like growth factor 1 receptor (IGF-1R) expression in breast cancer tissue and adjacent non-neoplastic breast tissue (ANCT) have produced conflicting results. The IGF-1 and IGF-1R expression in pairs of breast cancer tissue and ANCT were investigated using RT-PCR and immunohistochemistry. The results of both methods were compared. Materials and Methods: IGF-1 and IGF-1R mRNA from 31 specimen pairs were estimated using RT-PCR. Immunohistochemistry for IGF-1R was carried out on 20 specimen pairs and the strength of staining was scored. Results: The mean relative IGF-1 mRNA level was lower in the cancerous tissue (mean 0.450±0.206) than in the ANCT (mean 0.632±0.384) (paired t-test, p=0.001). There was no measurable difference in relative IGF-1R mRNA levels in the cancerous tissue (mean 0.146±0.08) and the ANCT (mean 0.14608±0.108) (paired t-test, p=0.807). Using immunohistochemistry, there was no statistical difference (paired t-test, p=0.910) in IGF-1R staining scores between cancer (mean 1.93) and ANCT (mean 1.90). The comparison between the two methodologies showed no correlation (Pearson's Correlation Coefficient=-0.393). Discussion: It can be concluded that IGF-1 expression is lower in cancerous tissue, thus supporting a paracrine relationship between cancerous tissue and ANCT, which may be useful in the prevention, diagnosis and treatment of breast cancer. There was no difference in the expression of the IGF-1 receptor in both types of tissue, as proven by RT-PCR and immunohistochemistry. Conflicting results in previous studies may be due to the different methods used to measure IGF-1R expression. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved %U https://ar.iiarjournals.org/content/anticanres/26/1A/167.full.pdf