RT Journal Article
SR Electronic
T1 The Role of Inflammation-associated microRNA-4257 as a Promoter of Malignancy in Colorectal Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3349
OP 3360
DO 10.21873/anticanres.15822
VO 42
IS 7
A1 SATOSHI KATAOKA
A1 TOMOHIRO ARITA
A1 HIROTAKA KONISHI
A1 YUSUKE YAMAMOTO
A1 RIE SHIBATA
A1 TAIGA YAMAMOTO
A1 JUN SHIBAMOTO
A1 HIROTAKA FURUKE
A1 KAZUYA TAKABATAKE
A1 WATARU TAKAKI
A1 JUN KIUCHI
A1 HIROKI SHIMIZU
A1 SHUHEI KOMATSU
A1 ATSUSHI SHIOZAKI
A1 YOSHIAKI KURIU
A1 EIGO OTSUJI
YR 2022
UL http://ar.iiarjournals.org/content/42/7/3349.abstract
AB Background/Aim: The clinical significance ofmiR-4257 in patients with colorectal cancer (CRC) remains unclear. Here, we investigated the usefulness of measuring miR-4257 levels in the plasma and cancer tissues of patients with CRC, and the function of miR-4257 in CRC cell lines. Materials and Methods: miR-4257 levels were measured in the plasma and cancer tissues of patients with CRC using quantitative polymerase chain reaction. The relationships between miR-4257 level and clinicopathological features were examined. Proliferation, transwell, wound healing, and adhesion assays were performed using a miR-4257 mimic and inflammatory cytokines. Results: Relapse-free survival was significantly lower in patients with high miR-4257 levels in the plasma and cancer tissue (p<0.001 andp=0.016, respectively). High miR-4257 expression was an independent predictive factor for recurrence (p=0.017 and p=0.028). Addition of inflammatory cytokines to CRC and normal cell lines increased the expression of miR-4257 in the cell lines and cell culture medium. Over-expression of miR-4257 in CRC cells increased malignancy, while over-expression in normal cells increased adhesion to CRC cells. The addition of inflammatory cytokines to normal cell lines enhanced adhesion to CRC cell lines. Conclusion: miR-4257 level in plasma and cancer tissues is a biomarker of disease recurrence in patients with CRC. Moreover, miR-4257 promoted tumour growth and was associated with cancer-induced inflammation.