PT - JOURNAL ARTICLE AU - DAISAKU TODA AU - TETSUYA OTA AU - KAZUNORI TSUKUDA AU - KEITARO WATANABE AU - TOSHIYUKI FUJIYAMA AU - MASAKAZU MURAKAMI AU - MINORU NAITO AU - NOBUYOSHI SHIMIZU TI - Gefitinib Decreases the Synthesis of Matrix Metalloproteinase and the Adhesion to Extracellular Matrix Proteins of Colon Cancer Cells DP - 2006 Jan 01 TA - Anticancer Research PG - 129--134 VI - 26 IP - 1A 4099 - http://ar.iiarjournals.org/content/26/1A/129.short 4100 - http://ar.iiarjournals.org/content/26/1A/129.full SO - Anticancer Res2006 Jan 01; 26 AB - Background: Adhesion to extracellular matrix (ECM) proteins and degradation of basement membranes by matrix metalloproteinase (MMP) play important roles in cancer metastasis. In this study, the effects of gefitinib on the enzymatic activity of MMP and adhesion to ECM proteins in the HT29 colon cancer cell line were investigated. Materials and Methods: Microtiter plates, coated with ECM proteins, were used to investigate the adhesion of cancer cells to ECM proteins. The expression of MMPs was examined by zymography and semi-quantitative RT-PCR. Results: Gefitinib inhibited MMP-9 and MMP-2 secretion and mRNA expression in HT29 cells. Gefitinib also reduced the ability to adhere to laminin and type IV collagen. These effects were observed at such low doses that gefitinib had neither an antiproliferative effect nor the ability to induce apoptosis. Conclusion: Gefitinib decreased the production of MMPs and the adhesion to ECM proteins, important steps associated with cancer metastasis. These results suggest that gefitinib may have antimetastatic activity in colon cancer. Copyright© 2006 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved