RT Journal Article
SR Electronic
T1 Purification, Characterization and Biological Significance of Tumor-derived Exosomes
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3703
OP 3707
VO 25
IS 6A
A1 KOGA, KENICHIRO
A1 MATSUMOTO, KOTARO
A1 AKIYOSHI, TAKASHI
A1 KUBO, MAKOTO
A1 YAMANAKA, NAOKI
A1 TASAKI, AKIRA
A1 NAKASHIMA, HIROSHI
A1 NAKAMURA, MASAFUMI
A1 KUROKI, SYOJI
A1 TANAKA, MASAO
A1 KATANO, MITSUO
YR 2005
UL http://ar.iiarjournals.org/content/25/6A/3703.abstract
AB Exosomes are nanovesicles that are released into the extracellular environment during the fusion of multivesicular bodies with the plasma membrane. Exosomes released from dendritic cells, dexosomes, have several biological functions, for example as immunostimulants. Some tumor cells also secrete exosomes (Tu-exosomes). Although experimental data obtained with the use of dexosomes suggest a biological function of Tu-exosomes, this still remains poorly understood. To examine the function of Tu-exosomes, we established a method for collecting highly purified Tu-exosomes, using paramagnetic beads coated with antibodies against tumor-specific proteins such as HER2/neu. With these antibody-coated beads (Ab-beads), it was possible to collect HER2-expressing Tu-exosomes of high purity. Tu-exosomes were also collected from malignant ascites, which contain exosomes secreted from various types of cells such as tumor cells, lymphoid cells and mesothelial cells. The isolation of Tu-exosomes was confirmed by FACS analysis. With regard to their biological functions, Tu-exosomes cultured with a human breast cancer cell line bound to the cell surface and increased tumor cell proliferation. These data indicate that Tu-exosomes may have physiological functions. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved