PT  - JOURNAL ARTICLE
AU  - KOGA, KENICHIRO
AU  - MATSUMOTO, KOTARO
AU  - AKIYOSHI, TAKASHI
AU  - KUBO, MAKOTO
AU  - YAMANAKA, NAOKI
AU  - TASAKI, AKIRA
AU  - NAKASHIMA, HIROSHI
AU  - NAKAMURA, MASAFUMI
AU  - KUROKI, SYOJI
AU  - TANAKA, MASAO
AU  - KATANO, MITSUO
TI  - Purification, Characterization and Biological Significance of Tumor-derived Exosomes
DP  - 2005 Nov 01
TA  - Anticancer Research
PG  - 3703--3707
VI  - 25
IP  - 6A
4099  - http://ar.iiarjournals.org/content/25/6A/3703.short
4100  - http://ar.iiarjournals.org/content/25/6A/3703.full
SO  - Anticancer Res2005 Nov 01; 25
AB  - Exosomes are nanovesicles that are released into the extracellular environment during the fusion of multivesicular bodies with the plasma membrane. Exosomes released from dendritic cells, dexosomes, have several biological functions, for example as immunostimulants. Some tumor cells also secrete exosomes (Tu-exosomes). Although experimental data obtained with the use of dexosomes suggest a biological function of Tu-exosomes, this still remains poorly understood. To examine the function of Tu-exosomes, we established a method for collecting highly purified Tu-exosomes, using paramagnetic beads coated with antibodies against tumor-specific proteins such as HER2/neu. With these antibody-coated beads (Ab-beads), it was possible to collect HER2-expressing Tu-exosomes of high purity. Tu-exosomes were also collected from malignant ascites, which contain exosomes secreted from various types of cells such as tumor cells, lymphoid cells and mesothelial cells. The isolation of Tu-exosomes was confirmed by FACS analysis. With regard to their biological functions, Tu-exosomes cultured with a human breast cancer cell line bound to the cell surface and increased tumor cell proliferation. These data indicate that Tu-exosomes may have physiological functions. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved