TY - JOUR T1 - P16<sup>INK4a</sup> Correlates to Human Papillomavirus Presence, Response to Radiotherapy and Clinical Outcome in Tonsillar Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 4375 LP - 4383 VL - 25 IS - 6C AU - HANNA MELLIN DAHLSTRAND AU - DAVID LINDQUIST AU - LINDA BJÖRNESTAL AU - ANN OHLSSON AU - TINA DALIANIS AU - EVA MUNCK-WIKLAND AU - GÖRAN ELMBERGER Y1 - 2005/11/01 UR - http://ar.iiarjournals.org/content/25/6C/4375.abstract N2 - Background: Human papillomavirus (HPV) in tonsillar carcinoma is correlated with favourable clinical outcome. Here, p16INK4A, in situ HPV DNA hybridisation (ISH) and HPV L1 capsid detection were evaluated in tonsillar carcinoma to predict the response to radiotherapy (RT) and prognosis. Materials and Methods: Fifty-one pre-treatment paraffin-embedded tonsillar cancer biopsies were analysed. Immunohistochemistry (IHC) was used for p16INK4A and HPV L1 capsid analysis and PCR and ISH for HPV detection. Results: High-risk HPV DNA was detected by PCR in 49% of the tumours. P16INK4a staining was correlated to HPV. In the high-grade p16INK4a staining group, 94% had a complete RT response. High p16INK4a staining as well as the HPV PCR-positive cases had a favourable prognosis. HPV DNA ISH and L1 IHC could not predict RT response or clinical outcome. Conclusion: P16INK4a overexpression was correlated to HPV in tonsillar carcinoma and is useful for predicting RT response and prognosis in tonsillar carcinoma patients. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -