@article {TAKAHASHI1307, author = {SHUNSUKE TAKAHASHI and TAKAHISA SHINOMIYA and YUKITOSHI NAGAHARA}, title = {Azoxystrobin Induces Apoptosis and Cell Cycle Arrest in Human Leukemia Cells Independent of p53 Expression}, volume = {42}, number = {3}, pages = {1307--1312}, year = {2022}, doi = {10.21873/anticanres.15598}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Azoxystrobin (AZOX), a methoxyacrylate derivative, has potent antimicrobial and antitumor activities. Here, we report the anticancer effects of AZOX on the p53-negative human myelogenous leukemia cell line HL-60RG and the p53 positive human T-cell leukemia cell line MOLT-4F. Materials and Methods: Using both leukemia cells, the anticancer effect of AZOX treatment was analyzed throughout the cell cycle. Results: AZOX damaged both cell lines dose-dependently, and the cell damage rates were almost the same in both lines. Cell cycle distribution analysis showed that the treated MOLT-4F cells arrested at the S phase, whereas HL-60RG cells increased during the subG1 phase, suggesting that cell death was occurring. AZOX-induced cell death in HL-60RG was inhibited with the addition of uridine, which is used as a substrate for the salvage pathway of pyrimidine nucleotides. Conclusion: AZOX has p53-independent anticancer effects in leukemia cells, but the mechanisms underlying the damage differ between cell lines.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/42/3/1307}, eprint = {https://ar.iiarjournals.org/content/42/3/1307.full.pdf}, journal = {Anticancer Research} }