@article {STELLER1295, author = {DANIEL STELLER and ALEXANDRA SCHEIBERT and TABEA STURMHEIT and DOROTHEE ROSE and SAMER G. HAKIM}, title = {Impact of PRP and PRF on Viability of Zoledronic Acid Treated Osteoblasts in 2D and 3D Cell Culture}, volume = {42}, number = {3}, pages = {1295--1299}, year = {2022}, doi = {10.21873/anticanres.15596}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: Zoledronic acid (ZA) treatment of in vitro cultured osteoblasts (OB) results in reduction in viability, proliferation and differentiation. These effects are slightly attenuated when platelets-rich fibrin and plasma (PRF and PRP) are added. However, it is still unknown whether application of PRP/PRF on ZA-treated OB in a 3D-environment would influence the viability in relation to 2D-cultivation. Materials and Methods: Non-treated and ZA-treated OB were cultivated in 2D conditions or seeded in a 3D collagen scaffold with and without PRP/PRF. MTT test was carried out after 5 days of colonization. 4,6-diamidino-2{\textquoteright}-phenylindole, dihydrochloride (DAPI)-staining was performed in OB grown in 3D scaffolds to ensure spatial distribution of OB. Results: ZA led to a significant reduction in cell viability compared to the control group. Addition of either PRF or PRP to the 3D colonized and ZA-treated OB significantly enhanced their survival and viability in relation to 2D monolayer cultivation. Conclusion: The use of 3D-scaffolds has a positive effect on OB viability, and stimulation by PRF and PRP may provide a therapeutic approach to transfer these results into clinical routine for the treatment of patients with bisphosphonate related osteonecrosis of the jaw (BR-ONJ).}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/42/3/1295}, eprint = {https://ar.iiarjournals.org/content/42/3/1295.full.pdf}, journal = {Anticancer Research} }