RT Journal Article
SR Electronic
T1 Identification of Breast Cancer Stem Cells Using a Newly Developed Long-acting Fluorescence Probe, C5S-A, Targeting ALDH1A1
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 1199
OP 1205
DO 10.21873/anticanres.15586
VO 42
IS 3
A1 AOI OKAMOTO
A1 YOHEI FUNAKOSHI
A1 MASAHIRO OE
A1 RYO TAKAI
A1 HIROTAKA SUTO
A1 YOSHIAKI NAGATANI
A1 MEIKO NISHIMURA
A1 YOSHINORI IMAMURA
A1 TOMONARI KUNIHISA
A1 NAOMI KIYOTA
A1 KOJI MIKI
A1 KOUICHI OHE
A1 HIROKAZU TANINO
A1 HIRONOBU MINAMI
YR 2022
UL http://ar.iiarjournals.org/content/42/3/1199.abstract
AB Background/Aim: Aldehyde dehydrogenase (ALDH) 1A1 is a well-known marker for cancer stem cells (CSCs), characterized by self-renewal capacity and multidrug resistance in breast cancer. We developed a near-infrared turn-on fluorescence probe for ALDH1A1, C5S-A, which is suitable for observing and analyzing viable cells. Here, we demonstrated the utility of C5S-A in CSC research using breast cancer cell lines. Materials and Methods: To evaluate concordance between C5S-A and conventional stem cell markers, breast cancer cells sorted for ALDEFLUOR-positive cells and for CD44+/CD24– cell populations were stained with C5S-A. Tumorigenicity of C5S-A-positive cells was examined by mammosphere formation assay and subcutaneous transplantation to immunodeficient mice. Additionally, to determine how long fluorescence from a single staining remained observable, we cultured breast cancer cells for 5 days after C5S-A staining. We then evaluated whether C5S-A-positive cells possessed resistance to cytotoxic drugs by chronological imaging. Results: C5S-A staining showed good concordance with conventional breast CSC markers, and good utility for research into CSC characteristics in breast cancer cell lines, including tumorigenesis. Additionally, C5S-A was observable for more than 3 days with a single staining. Using this property, we then confirmed that C5S-A-positive cells possessed resistance to cytotoxic drugs, which is one of the characteristics of CSCs. Conclusion: We showed that C5S-A is suitable for CSC research using breast cancer cell lines, and confirmed its utility in observing cells over time.