RT Journal Article SR Electronic T1 Pharmacological Modulation of Lung Cancer Cells for Potassium Ion Depletion JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2609 OP 2616 VO 25 IS 4 A1 BRITTA ANDERSSON A1 PARVIZ BEHNAM-MOTLAGH A1 ROGER HENRIKSSON A1 KJELL GRANKVIST YR 2005 UL http://ar.iiarjournals.org/content/25/4/2609.abstract AB Background: Depletion of intracellular potassium ions (K+) is necessary for cells to shrink, induce DNA fragmentation and activate caspases, events which are features of apoptosis. Materials and Methods: We used 86Rb+ as a K+ analogue to evaluate the possibility of pharmacologically depleting human pulmonary mesothelioma (P31) and small cell lung cancer (U1690) cells of K+, for future use in studies of apoptosis induction. Results: The Na+, K+, 2Cl--cotransport inhibitor bumetanide transiently inhibited 86Rb+ influx, but when combined with the Na+, K+, ATPase pump inhibitor ouabain there was a marked and lasting (up to 6 h) 86Rb+ influx inhibition. Cellular K+ efflux was augmented by amphotericin B, digitonin and nigericin. Amphotericin B was an effective 86Rb+ efflux stimulator with low cytotoxicity, whereas digitonin caused cell detachment and nigericin increased LDH release in the U1690 cell line, indicating considerable toxicity of the drugs. Conclusion: It is possible to efficiently reduce intracellular K+ by persistent K+ influx inhibition and simultaneous K+ efflux stimulation with clinically available drugs. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved