TY - JOUR T1 - Snail Mucus Enhances Chemosensitivity of Triple-negative Breast Cancer <em>Via</em> Activation of the Fas Pathway JF - Anticancer Research JO - Anticancer Res SP - 845 LP - 855 DO - 10.21873/anticanres.15542 VL - 42 IS - 2 AU - CHIEN-YI HO AU - DAI-WEI HU AU - BO-RONG CHEN AU - CHUN-CHUN YANG AU - CHUN-HSU YAO AU - TIEN NI AU - WEI-CHUAN HO AU - YUEH-SHENG CHEN AU - CHIH-YEN TU AU - WEI-CHAO CHANG AU - YANG-CHANG WU AU - CHIH-HSIN TANG AU - HUI-CHI HUANG AU - WEI-CHIEN HUANG AU - TZONG-SHIUN LI Y1 - 2022/02/01 UR - http://ar.iiarjournals.org/content/42/2/845.abstract N2 - Background/Aim: The poor prognosis and chemoresistance of patients with triple-negative breast cancer (TNBC) urge the development of new therapeutic strategies. Snail mucus has shown its ability against inflammation, a process closely related to tumorigenesis, suggesting a potential anti-cancer activity. Materials and Methods: The effect and mechanisms of snail mucus on cell viability were determined by IncuCyte Live-cell analysis and molecular biological methods. The anti-cancer fractions of snail mucus were isolated and identified by medium pressure liquid chromatography (MPLC) and nuclear magnetic resonance (NMR) spectrometry analysis. Results: Snail mucus significantly decreased the viability of TNBC cells with relatively lower cytotoxicity to normal breast epithelial cells and enhanced their response to chemotherapy through activation of Fas signaling by suppressing nucleolin. Two peptide fractions have been identified as the anti-cancer ingredients of the snail mucus. Conclusion: Snail mucus can induce programmed cell death via the extrinsic apoptotic pathway and has therapeutic potential by achieving a chemo-sensitizing effect in TNBCs. ER -