@article {HO845, author = {CHIEN-YI HO and DAI-WEI HU and BO-RONG CHEN and CHUN-CHUN YANG and CHUN-HSU YAO and TIEN NI and WEI-CHUAN HO and YUEH-SHENG CHEN and CHIH-YEN TU and WEI-CHAO CHANG and YANG-CHANG WU and CHIH-HSIN TANG and HUI-CHI HUANG and WEI-CHIEN HUANG and TZONG-SHIUN LI}, title = {Snail Mucus Enhances Chemosensitivity of Triple-negative Breast Cancer Via Activation of the Fas Pathway}, volume = {42}, number = {2}, pages = {845--855}, year = {2022}, doi = {10.21873/anticanres.15542}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: The poor prognosis and chemoresistance of patients with triple-negative breast cancer (TNBC) urge the development of new therapeutic strategies. Snail mucus has shown its ability against inflammation, a process closely related to tumorigenesis, suggesting a potential anti-cancer activity. Materials and Methods: The effect and mechanisms of snail mucus on cell viability were determined by IncuCyte Live-cell analysis and molecular biological methods. The anti-cancer fractions of snail mucus were isolated and identified by medium pressure liquid chromatography (MPLC) and nuclear magnetic resonance (NMR) spectrometry analysis. Results: Snail mucus significantly decreased the viability of TNBC cells with relatively lower cytotoxicity to normal breast epithelial cells and enhanced their response to chemotherapy through activation of Fas signaling by suppressing nucleolin. Two peptide fractions have been identified as the anti-cancer ingredients of the snail mucus. Conclusion: Snail mucus can induce programmed cell death via the extrinsic apoptotic pathway and has therapeutic potential by achieving a chemo-sensitizing effect in TNBCs.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/42/2/845}, eprint = {https://ar.iiarjournals.org/content/42/2/845.full.pdf}, journal = {Anticancer Research} }