RT Journal Article SR Electronic T1 Patterns of Tumor Vasculogenesis on a Chick Embryo Chorioallantoic Membrane In Vivo Model of Breast Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 877 OP 883 DO 10.21873/anticanres.15545 VO 42 IS 2 A1 COMSA, SERBAN A1 CEAUSU, AMALIA-RALUCA A1 POPESCU, ROXANA A1 CIMPEAN, ANCA-MARIA A1 SARB, SIMONA A1 RAICA, MARIUS YR 2022 UL http://ar.iiarjournals.org/content/42/2/877.abstract AB Background/Aim: Understanding tumor vasculogenesis is a cornerstone for the inhibition of tumor progression. This study aimed to generate an in vivo breast cancer environment to analyze the patterns of tumor vasculogenesis. Materials and Methods: Human mesenchymal stem cells (hMSC) and breast cancer MCF-7 cells (MCF-7) were seeded onto a chorioallantoic membrane (CAM) and, after a 7-day incubation, we performed a morphological and immunohistochemical analysis of CAM. Results: hMSC and MCF-7 activated vasculogenesis and hematopoiesis on CAM. They stimulated the development of cord/capillary-like structures (CLS), formed by endothelial-like cells and hematopoietic cells. CLS presented a polygonal pattern, evolving towards a clearly visible plexus. Immunohistochemically, CLS were CD105+/AC133+/Oct3/4+, and the intensity was weak-moderate in the endothelial-like cells (inconstant) and weak in the hematopoietic cells. Conclusion: Tumor and embryonic vasculogenesis share a common paradigm, while CD105, AC133, and Oct3/4 were found to play a role in establishing the vasculogenic and hematopoietic stage.