PT  - JOURNAL ARTICLE
AU  - COMSA, SERBAN
AU  - CEAUSU, AMALIA-RALUCA
AU  - POPESCU, ROXANA
AU  - CIMPEAN, ANCA-MARIA
AU  - SARB, SIMONA
AU  - RAICA, MARIUS
TI  - Patterns of Tumor Vasculogenesis on a Chick Embryo Chorioallantoic Membrane <em>In Vivo</em> Model of Breast Cancer
AID  - 10.21873/anticanres.15545
DP  - 2022 Feb 01
TA  - Anticancer Research
PG  - 877--883
VI  - 42
IP  - 2
4099  - http://ar.iiarjournals.org/content/42/2/877.short
4100  - http://ar.iiarjournals.org/content/42/2/877.full
SO  - Anticancer Res2022 Feb 01; 42
AB  - Background/Aim: Understanding tumor vasculogenesis is a cornerstone for the inhibition of tumor progression. This study aimed to generate an in vivo breast cancer environment to analyze the patterns of tumor vasculogenesis. Materials and Methods: Human mesenchymal stem cells (hMSC) and breast cancer MCF-7 cells (MCF-7) were seeded onto a chorioallantoic membrane (CAM) and, after a 7-day incubation, we performed a morphological and immunohistochemical analysis of CAM. Results: hMSC and MCF-7 activated vasculogenesis and hematopoiesis on CAM. They stimulated the development of cord/capillary-like structures (CLS), formed by endothelial-like cells and hematopoietic cells. CLS presented a polygonal pattern, evolving towards a clearly visible plexus. Immunohistochemically, CLS were CD105+/AC133+/Oct3/4+, and the intensity was weak-moderate in the endothelial-like cells (inconstant) and weak in the hematopoietic cells. Conclusion: Tumor and embryonic vasculogenesis share a common paradigm, while CD105, AC133, and Oct3/4 were found to play a role in establishing the vasculogenic and hematopoietic stage.