TY - JOUR T1 - CRMP4 Up-regulates M2 Macrophages and Myeloid-derived Suppressor Cells to Promote Pancreatic Cancer in Mice JF - Anticancer Research JO - Anticancer Res SP - 791 LP - 799 DO - 10.21873/anticanres.15537 VL - 42 IS - 2 AU - YUZO MINEGISHI AU - YUKIHIKO HIROSHIMA AU - KEIICHI YAZAWA AU - SHO SATO AU - YASUHIRO YABUSHITA AU - YUKI HOMMA AU - RYUSEI MATSUYAMA AU - IKUMA KATO AU - YOSHIO GOSHIMA AU - ITARU ENDO Y1 - 2022/02/01 UR - http://ar.iiarjournals.org/content/42/2/791.abstract N2 - Background/Aim: We previously observed higher prevalence of high-grade pancreatic intraepithelial neoplasia (PanIN) in LSL-KrasG12D/+; Pdx1Cre/+ (KC-Crmp4wild) mice than LSL-KrasG12D/+; Pdx1Cre/+; Crmp4–/– (KC-Crmp4–/–) mice. This study investigated the relationship between collapsin response mediator protein 4 (CRMP4) and immune cell infiltration in pancreatic cancer. Materials and Methods: PanIN was induced by intraperitoneal injection of caerulein into KC-Crmp4wild and KC-Crmp4–/– mice, and immune cells in PanIN lesions were compared. Subcutaneous tumors were created by injecting Pan02 cells, and tumor diameter was compared between Crmp4wild and Crmp4–/– mice every 7 days. Peritumoral immune cells were examined immunohisto chemically. Results: High-grade PanIN in KC mice showed statistically significantly high expression of CD163 (p=0.031) and CD11b (p=0.027). Following subcutaneous injection of Pan02 cells, tumor diameter was greater in Crmp4wild mice than Crmp4–/– mice. Crmp4wild mice exhibited higher CD163 and CD11b expression than Crmp4–/– mice in tumors (p<0.001). Conclusion: CRMP4 might promote pancreatic cancer by up-regulating M2 macrophages and myeloid-derived suppressor cells. ER -