RT Journal Article
SR Electronic
T1 Dipeptidyl Peptidase-4 from Cancer-associated Fibroblasts Stimulates the Proliferation of Scirrhous-type Gastric Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 501
OP 509
DO 10.21873/anticanres.15508
VO 42
IS 1
A1 SHUHEI KUSHIYAMA
A1 MASAKAZU YASHIRO
A1 YURIE YAMAMOTO
A1 TOMOHIRO SERA
A1 ATSUSHI SUGIMOTO
A1 SADAAKI NISHIMURA
A1 SHINGO TOGANO
A1 KENJI KURODA
A1 TOMOHISA OKUNO
A1 YUICHIRO MIKI
A1 MASAICHI OHIRA
YR 2022
UL http://ar.iiarjournals.org/content/42/1/501.abstract
AB Background/Aim: Cancer-associated fibroblasts (CAFs) may promote the malignancy of human scirrhous gastric cancer (SGC) cells. We conducted the present study to identify novel growth factors from CAFs. Materials and Methods: OCUM-12 and 2 CAF cell lines were used. The proliferation of cancer cells was determined by the number of cancer cells or the MTT assay. The growth factor(s) were purified and characterized by the gel filtration chromatography and protein array. Results: The molecular weight of the growth-stimulating factor was estimated to be approximately 66–669 kDa. Protein array of conditioned medium (CM) from CAFs indicated that dipeptidyl peptidase-4 (DPP-4) was one of the growth factors. The addition of CM increased the phosphorylation of C-X-C chemokine receptor 4 (CXCR4). The DPP-4 inhibitor significantly inhibited the growth-stimulating activity of CM. Conclusion: DPP-4 from CAFs might be one of the growth-stimulating factors for SGC through CXCR4.