TY - JOUR T1 - (-)-Menthol Inhibits DNA Topoisomerases I, II α and β and Promotes NF-Î B expression in Human Gastric Cancer SNU-5 Cells JF - Anticancer Research JO - Anticancer Res SP - 2069 LP - 2074 VL - 25 IS - 3B AU - JING-PIN LIN AU - HSU-FENG LU AU - JAU-HONG LEE AU - JAUNG-GUNG LIN AU - TE-CHUN HSIA AU - LII-TZU WU AU - JING-GUNG CHUNG Y1 - 2005/05/01 UR - http://ar.iiarjournals.org/content/25/3B/2069.abstract N2 - It has been reported that (-)-Menthol can inhibit the growth of rat liver epithelial tumor cells and is a potent chemopreventive agent. The purpose of the present experiment was to examine and identify cellular processes leading to cell death which are affected by (-)-Menthol in human gastric SNU-5 cancer cells. Cell death (cytotoxicity) was examined and analyzed by trypan blue stain and flow cytometric methods. It was shown that (-)-Menthol inhibited the proliferation of the cells in a dose- and time-dependent manner, inhibited topoisomerase I, IIα and IIβ, but promoted the levels of NF-Î B gene expression based on the Western blot and polymerase chain reaction (PCR) and cDNA microarray methods. These data suggest that (-)-Menthol may induce cytotoxicity through inhibiting gene expression of topoisomerase I, IIα and IIβ and promoting the gene expression of NF-Î B in SNU-5 cells. Copyright© 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved ER -